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smfp flag fragment  (Addgene inc)


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    Structured Review

    Addgene inc smfp flag fragment
    Smfp Flag Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smfp+flag+fragment/pCAG_smFP+FLAG+(Plasmid+%2359756)/pm33558651-61-30-47
    Average 93 stars, based on 6 article reviews
    smfp flag fragment - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Defective AMPA-mediated synaptic transmission and morphology in human neurons with hemizygous SHANK3 deletion engrafted in mouse prefrontal cortex.
    Article Snippet: The deletion was verified using PCR product purification, subcloning into a TOPO TA cloning vector (Thermo Fisher, Cat# 450030), and sequencing. .. The following lenti vectors were used in this study: pBOBsynP-HTB (Addgene, Cat#30195) [35]; CamKIIα:smFPTVA-RVG (constructed in the lab by subcloning the following fragments into the pLenti_CaMKIIα_GFP vector [Addgene plasmid #96941]; smFP-Flag fragment from the pCAG_smFP-Flag_dark vector [a gift fromMegan Williams] [36]; F2A-TVA-T2A fragment from the pBOB_synP-HTB vector (Addgene, Cat#30195); oG fragment from the pCAG_oG vector [Addgene plasmid #74288] [37]); CamKIIα: GFP-TVA-RGV (constructed in the lab using the CamKIIα: smFP-TVA-RGV vector and substituting the smFP-Flag fragment with smFP-Flag_bright fragment from the pCAG_ smFP-Flag_membrane_bright vector [a gift from Megan Williams] [36]). ..

    Subcloning:

    Article Title: Defective AMPA-mediated synaptic transmission and morphology in human neurons with hemizygous SHANK3 deletion engrafted in mouse prefrontal cortex.
    Article Snippet: The deletion was verified using PCR product purification, subcloning into a TOPO TA cloning vector (Thermo Fisher, Cat# 450030), and sequencing. .. The following lenti vectors were used in this study: pBOBsynP-HTB (Addgene, Cat#30195) [35]; CamKIIα:smFPTVA-RVG (constructed in the lab by subcloning the following fragments into the pLenti_CaMKIIα_GFP vector [Addgene plasmid #96941]; smFP-Flag fragment from the pCAG_smFP-Flag_dark vector [a gift fromMegan Williams] [36]; F2A-TVA-T2A fragment from the pBOB_synP-HTB vector (Addgene, Cat#30195); oG fragment from the pCAG_oG vector [Addgene plasmid #74288] [37]); CamKIIα: GFP-TVA-RGV (constructed in the lab using the CamKIIα: smFP-TVA-RGV vector and substituting the smFP-Flag fragment with smFP-Flag_bright fragment from the pCAG_ smFP-Flag_membrane_bright vector [a gift from Megan Williams] [36]). ..

    Plasmid Preparation:

    Article Title: Defective AMPA-mediated synaptic transmission and morphology in human neurons with hemizygous SHANK3 deletion engrafted in mouse prefrontal cortex.
    Article Snippet: The deletion was verified using PCR product purification, subcloning into a TOPO TA cloning vector (Thermo Fisher, Cat# 450030), and sequencing. .. The following lenti vectors were used in this study: pBOBsynP-HTB (Addgene, Cat#30195) [35]; CamKIIα:smFPTVA-RVG (constructed in the lab by subcloning the following fragments into the pLenti_CaMKIIα_GFP vector [Addgene plasmid #96941]; smFP-Flag fragment from the pCAG_smFP-Flag_dark vector [a gift fromMegan Williams] [36]; F2A-TVA-T2A fragment from the pBOB_synP-HTB vector (Addgene, Cat#30195); oG fragment from the pCAG_oG vector [Addgene plasmid #74288] [37]); CamKIIα: GFP-TVA-RGV (constructed in the lab using the CamKIIα: smFP-TVA-RGV vector and substituting the smFP-Flag fragment with smFP-Flag_bright fragment from the pCAG_ smFP-Flag_membrane_bright vector [a gift from Megan Williams] [36]). ..



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